MYOZ3 Polyklonaler Antikörper

MYOZ3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 23880-1-AP

Synonyme

Calsarcin 3, CS 3, CS3, FATZ related protein 3, FRP3, MYOZ3, myozenin 3

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Galerie der Validierungsdaten

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  WB analysis of rat skeletal muscle using 23880-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 23880-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23880-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 23880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Skelettmuskelgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23880-1-AP bindet in WB, IHC,ELISA MYOZ3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOZ3 fusion protein Ag20839
• Vollständiger Name: myozenin 3
• Berechnetes Molekulargewicht: 251 aa, 27 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC074870
• Gene symbol: MYOZ3
• Gene ID (NCBI): 91977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Martina (Verified Customer) (07-03-2023)

IF protocol for fixed cryosection of mouse skeletal muscle: after a quick permeabilization step with Triton 0,3% in PBS, block with BSA 0,5% and GS 10% in PBS for at least 2 hours at room temperature. Then incubate with primary antibody 1:100 in blocking solution overnight at +4°C. The day after, incubate with secondary antibody 1:200 in blocking solution for 2 hours at room temperature. WB protocol for mouse skeletal muscle proteins: block the membrane with 5% milk in TBS-T for at least 2 hours at room temperature; then incubate with primary antibody 1:1000 in 5% milk in TBS-T at +4°C overnight. The day after, incubate with secondary antibody 1:2000 in 5% milk in TBS-T for 2 hours at room temperature. Developing the membrane, you will see double bands between 39 and 28 kDa.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: Murine skeletal muscle

MYOZ3 Antibody Western Blot,Immunofluorescence validation ( dilution) in Murine skeletal muscle (Cat no:23880-1-AP)