MYPN-Specific Polyklonaler Antikörper

MYPN-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16180-1-PBS

Synonyme

MYPN, MYOP, Myopalladin

---

• 

  WB analysis using 16180-1-AP (same clone as 16180-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16180-1-AP (same clone as 16180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYPN-Specific antibody (16180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

16180-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYPN-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myopalladin
• Berechnetes Molekulargewicht: 145 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: NM_032578
• Gene symbol: MYPN
• Gene ID (NCBI): 84665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.