MYPT1 Monoklonaler Antikörper

MYPT1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A1A9

Kat-Nr. : 66506-1-PBS

Synonyme

MBS, MYPT1, PPP1R12A

---

• 

  WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66506-1-Ig (same clone as 66506-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66506-1-Ig (same clone as 66506-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66506-1-Ig (same clone as 66506-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66506-1-PBS bindet in WB, IF/ICC, Indirect ELISA MYPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYPT1 fusion protein Ag17496
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Berechnetes Molekulargewicht: 1030 aa, 115 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC111752
• Gene symbol: MYPT1
• Gene ID (NCBI): 4659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.