MYSM1-Specific Polyklonaler Antikörper

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• KD/KO Validated

MYSM1-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20078-1-AP

Synonyme

MYSM1, KIAA1915, EC:3.4.19.-, Deubiquitinase MYSM1, 2A-DUB

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  WB analysis of A549 using 20078-1-AP

  WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.

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  WB analysis of HeLa using 20078-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20078-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 20078-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20078-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20078-1-AP

  IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 20078-1-AP

  IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:1000) with HeLa cells lysate 1200 ug.

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  IHC staining of mouse small intestine using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 20078-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MYSM1-Specific antibody (20078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Pankreaskarzinomgewebe, humanes Milzgewebe, humanes Urothelkarzinomgewebe, Maus-Dünndarmgewebe, Rattennierengewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

20078-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MYSM1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Myb-like, SWIRM and MPN domains 1
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: NM_001085487
• Gene symbol: MYSM1
• Gene ID (NCBI): 114803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.

Publikationen

Species	Application	Title
human	IHC	Cell Death Discov; MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.; Authors - Xiaolin Guan