MYT1L Polyklonaler Antikörper

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• KD/KO Validated

MYT1L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 25234-1-AP

Synonyme

NZF1, MyT1-L, MyT1 L, Myelin transcription factor 1-like protein, KIAA1106

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  WB analysis of HepG2 using 25234-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25234-1-AP (MYT1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25234-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25234-1-AP (MYT1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25234-1-AP (MYT1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25234-1-AP (MYT1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

25234-1-AP bindet in WB, IHC, IF, IP, ELISA MYT1L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYT1L fusion protein Ag18698
• Vollständiger Name: myelin transcription factor 1-like
• Berechnetes Molekulargewicht: 1186 aa, 133 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC137273
• Gene symbol: MYT1L
• Gene ID (NCBI): 23040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Neuron; A MYT1L syndrome mouse model recapitulates patient phenotypes and reveals altered brain development due to disrupted neuronal maturation.; Authors - Jiayang Chen
mouse	IF	Cell Prolif; Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors; Authors - Yuhang Huang
human	WB	Brain Res Bull; miRNA-338-3p inhibits glioma cell proliferation and progression by targeting MYT1L; Authors - Zhengtao Yu ; KD Validated
mouse	WB	J Biol Chem; Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis; Authors - Masayoshi Nagai
human	IF	Nat Neurosci; Age-related Huntington's disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy; Authors - Young Mi Oh
mouse	IP	Genome Res; MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain; Authors - Jiayang Chen