Maspin Monoklonaler Antikörper

Maspin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G5A3

Kat-Nr. : 67633-1-PBS

Synonyme

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

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  WB analysis using 67633-1-Ig (same clone as 67633-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

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  WB analysis of HUMAN saliva using 67633-1-Ig (same clone as 67633-1-PBS)

  Human saliva were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 67633-1-Ig (same clone as 67633-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 67633-1-Ig (same clone as 67633-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67633-1-Ig (same clone as 67633-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Maspin antibody (67633-1-Ig, Clone: 3G5A3 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67633-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Maspin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maspin fusion protein Ag19129
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC020713
• Gene symbol: Maspin
• Gene ID (NCBI): 5268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.