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MCP-1/CCL2 Rekombinanter Antikörper

MCP-1/CCL2 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230483F11

Kat-Nr. : 83157-5-PBS

Synonyme

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using MCP-1/CCL2 Recombinant antibody (83157-5-RR)

    WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

    Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83157-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83157-5-RR

    Affinity and Kinetic Characterization of 83157-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

Geprüfte Anwendungen

Produktinformation

83157-5-PBS bindet in WB, Indirect ELISA MCP-1/CCL2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name chemokine (C-C motif) ligand 2
Beobachtetes Molekulargewicht16 kDa
GenBank-ZugangsnummerNM-011333
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.