MCP-1/CCL2 Rekombinanter Antikörper

MCP-1/CCL2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

230483F11

Kat-Nr. : 83157-5-RR

Synonyme

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

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  WB analysis of RAW 264.7 using 83157-5-RR

  Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83157-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS und Brefeldin A behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

83157-5-RR bindet in WB, ELISA MCP-1/CCL2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: chemokine (C-C motif) ligand 2
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: NM-011333
• Gene symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publikationen

Species	Application	Title
human	WB	Environ Toxicol; Luteolin Inhibits Indoxyl Sulfate-Induced ICAM-1 and MCP-1 Expression by Inducing HO-1 Expression in EA.hy926 Human Endothelial Cells; Authors - Li-Chien Chang
mouse	WB	Research (Wash D C); Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44-AKT-CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation; Authors - Xi Jin