MLPH Monoklonaler Antikörper

MLPH Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A8D12

Kat-Nr. : 66092-1-PBS

Synonyme

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

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  WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66092-1-Ig (same clone as 66092-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66092-1-Ig (same clone as 66092-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66092-1-Ig (same clone as 66092-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66092-1-PBS bindet in WB, IHC, IF/ICC, ELISA MLPH und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MLPH fusion protein Ag19286
• Vollständiger Name: melanophilin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC001653
• Gene symbol: MLPH
• Gene ID (NCBI): 79083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.