Midkine Polyklonaler Antikörper

Midkine Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28546-1-AP

Synonyme

ARAP, FLJ27379, MDK, Midkine, MK, MK1, NEGF2

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  WB analysis using 28546-1-AP

  Mouse embryo tissues were subjected to SDS PAGE followed by western blot with 28546-1-AP (Midkine antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 28546-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 28546-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28546-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28546-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28546-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28546-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausembryogewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

28546-1-AP bindet in WB, IHC, ELISA Midkine und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Midkine fusion protein Ag29185
• Vollständiger Name: midkine (neurite growth-promoting factor 2)
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC011704
• Gene symbol: Midkine
• Gene ID (NCBI): 4192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	EBioMedicine; Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis; Authors - Masahiro Hashimoto
human	WB,IHC	Mol Ther Oncol; Comprehensive machine learning-based integration develops a novel prognostic model for glioblastoma; Authors - Qian Jiang
	WB	ACS Nano; Precise Photodynamic Therapy by Midkine Nanobody-Engineered Nanoparticles Remodels the Microenvironment of Pancreatic Ductal Adenocarcinoma and Potentiates the Immunotherapy; Authors - Chengming Qu