Midkine Rekombinanter Antikörper

Midkine Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241880H1

Kat-Nr. : 84815-1-PBS

Synonyme

MDK, 241880H1, Amphiregulin-associated protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

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  IHC staining of mouse embryo using 84815-1-RR (same clone as 84815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 84815-1-RR (Midkine antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84815-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 84815-1-RR (same clone as 84815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 84815-1-RR (Midkine antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84815-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84815-1-RR (same clone as 84815-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Midkine antibody (84815-1-RR, Clone: 241880H1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84815-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84815-1-RR (same clone as 84815-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Midkine Recombinant antibody (84815-1-RR, Clone:241880H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84815-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84815-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84815-1-RR against Human Midkine were performed. The affinity constant is 35.9 nM.

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Geprüfte Anwendungen

Produktinformation

84815-1-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA Midkine und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Midkine fusion protein Ag24547
• Vollständiger Name: midkine (neurite growth-promoting factor 2)
• Berechnetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC011704
• Gene symbol: Midkine
• Gene ID (NCBI): 4192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.