Mmp9 Rekombinanter Antikörper

Mmp9 Rekombinant Antikörper für IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

230069F1

Kat-Nr. : 82854-5-RR

Synonyme

230069F1, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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  IHC staining of mouse spleen using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of RAW 264.7 using 82854-5-RR

  1x10^6 unstimulated or LPS and BFA treated RAW 264.7 cells were intracellularly stained with 0.13 ug Anti-Mouse Mmp9 (82854-5-RR, Clone:230069F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82854-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82854-5-RR against Human Mmp9 were performed. The affinity constant is 76.6 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): 100ng/ml LPS+BFA RAW 264.7 cells

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82854-5-RR bindet in IHC, FC (Intra), ELISA Mmp9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Mmp9 fusion protein Eg0452
• Vollständiger Name: matrix metallopeptidase 9
• Berechnetes Molekulargewicht: 81kd
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: NM_013599
• Gene symbol: Mmp9
• Gene ID (NCBI): 17395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publikationen

Species	Application	Title
mouse	FC (Intra)	Commun Biol; The crosstalk of monocyte-neutrophil in hair follicles regulates neutrophil transepidermal migration in contact dermatitis; Authors - Zhan Fan