Mmp9 Rekombinanter Antikörper

Mmp9 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230069G7

Kat-Nr. : 82854-8-PBS

Synonyme

230069G7, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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  WB analysis using 82854-8-RR (same clone as 82854-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82854-8-RR (Mmp9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82854-8-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82854-8-RR (same clone as 82854-8-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-8-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82854-8-RR (same clone as 82854-8-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-8-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 82854-8-RR (same clone as 82854-8-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-8-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 82854-8-RR (same clone as 82854-8-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-8-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82854-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82854-8-RR against Mouse Mmp9 were performed. The affinity constant is 1.38 nM.

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Geprüfte Anwendungen

Produktinformation

82854-8-PBS bindet in WB, IHC, Indirect ELISA Mmp9 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Mmp9 fusion protein Eg0452
• Vollständiger Name: matrix metallopeptidase 9
• Berechnetes Molekulargewicht: 81kd
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: NM_013599
• Gene symbol: Mmp9
• Gene ID (NCBI): 17395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).