Mmp9 Rekombinanter Antikörper

Mmp9 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

230069G7

Kat-Nr. : 82854-8-RR

Synonyme

230069G7, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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  WB analysis using 82854-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82854-8-RR (Mmp9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82854-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82854-8-RR against Mouse Mmp9 were performed. The affinity constant is 1.38 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

82854-8-RR bindet in WB, IHC, ELISA Mmp9 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Mmp9 fusion protein Eg0452
• Vollständiger Name: matrix metallopeptidase 9
• Berechnetes Molekulargewicht: 81kd
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: NM_013599
• Gene symbol: Mmp9
• Gene ID (NCBI): 17395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay; Authors - Ying Sun
mouse	IHC	Biochem Biophys Res Commun; Usefulness of serial in vivo imaging to directly assess the role of inflammation in thrombus resolution and organization; Authors - Aditya Adinata