Mono/Di/Tri-Methyl-Histone H3 (Lys4) Rekombinanter Antikörper

Mono/Di/Tri-Methyl-Histone H3 (Lys4) Rekombinant Antikörper für WB, IHC, Dot Blot, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Dot Blot, ELISA

Konjugation

Unkonjugiert

CloneNo.

241942E11

Kat-Nr. : 84908-1-RR

Synonyme

H3K4Me1/Me2/Me3, Me Histone H3, Me Histone H3 (Lys4), Me Histone H3 Lys4, Me Histone H3-Lys4

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  WB analysis using 84908-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84908-1-RR (Mono/Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84908-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-1-RR (Mono/Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84908-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-1-RR (Mono/Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84908-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-1-RR (Mono/Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84908-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-1-RR (Mono/Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Dot Blot experiment of peptide using 84908-1-RR

  Dot blot analysis was used to confirm the specificity of 84908-1-RR Mono/Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Plazenta-Gewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in Dot Blot: peptide

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84908-1-RR bindet in WB, IHC, Dot Blot, ELISA Mono/Di/Tri-Methyl-Histone H3 (Lys4) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.