Mono-Methyl-Histone H3 (Lys4) Rekombinanter Antikörper

Mono-Methyl-Histone H3 (Lys4) Rekombinant Antikörper für WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241940G8

Kat-Nr. : 84740-1-PBS

Synonyme

H3 Me Lys4, H3 MeK4, H3 Methyl K4, H3 Methyl Lys4, H3 Methyl Lysine4

---

• 

  WB analysis using 84740-1-RR (same clone as 84740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84740-1-RR (Mono-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84740-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84740-1-RR (same clone as 84740-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys4) antibody (84740-1-RR, Clone: 241940G8 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84740-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of peptide using 84740-1-RR (same clone as 84740-1-PBS)

  Dot blot analysis was used to confirm the specificity of 84740-1-RR Mono-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84740-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 84740-1-RR (same clone as 84740-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys4) (84740-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84740-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84740-1-PBS bindet in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Mono-Methyl-Histone H3 (Lys4) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.