Mouse IgG2b isotype control Monoklonaler Antikörper

Mouse IgG2b isotype control Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Megathura crenulata

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

11B8C4

Kat-Nr. : 66360-3-PBS

Synonyme

Mouse IgG2b

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  WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

  Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 66360-3-Ig (same clone as 66360-3-PBS)

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig, Clone: 11B8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD20 (65085-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66360-3-PBS bindet in WB, IHC, ELISA Mouse IgG2b isotype control und zeigt Reaktivität mit Megathura crenulata

• Getestete Reaktivität: Megathura crenulata
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Mouse IgG2b isotype control
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).