TNF Alpha Monoklonaler Antikörper

TNF Alpha Monoklonal Antikörper für Single Cell (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

Single Cell (Intra)

Konjugation

5CFLX Fluorescent Dye

CloneNo.

7B8A11

Kat-Nr. : G60291-1-5C

Synonyme

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

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  Single Cell Sequencing experiment G60291-1-5C on Resting PBMC

  G60291-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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  Single Cell Sequencing experiment G60291-1-5C on PBMC treated with 4hr LPS + TI

  G60291-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in Single Cell (Intra): 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Empfohlene Verdünnung

• SINGLE CELL (INTRA): SINGLE CELL (INTRA) : <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

G60291-1-5C bindet in Single Cell (Intra) TNF Alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNF Alpha fusion protein Ag11413
• Vollständiger Name: tumor necrosis factor (TNF superfamily, member 2)
• Berechnetes Molekulargewicht: 233 aa, 26 kDa
• GenBank-Zugangsnummer: BC028148
• Gene symbol: TNF-alpha
• Gene ID (NCBI): 7124
• Konjugation: 5CFLX Fluorescent Dye
• Form: Liquid
• Lagerungspuffer: PBS with 1mM EDTA and 0.09% sodium azide
• Lagerungsbedingungen: 2-8°C Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.