MBP Polyklonaler Antikörper

MBP Polyklonal Antikörper für IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10458

Synonyme

myelin basic protein

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  IF Staining of mouse brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

• 

  IF Staining of rat brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite® Plus 488 MBP antibody (CL488-10458) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1, red), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite® Plus 488 MBP antibody (CL488-10458) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1, red), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10458 bindet in IF-P MBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MBP fusion protein Ag0713
• Vollständiger Name: myelin basic protein
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 14-23 kDa
• GenBank-Zugangsnummer: BC008749
• Gene symbol: Myelin basic protein
• Gene ID (NCBI): 4155
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.