Myh9 Polyklonaler Antikörper

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• KD/KO Validated

Myh9 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14844-1-AP

Synonyme

C80049, D0Jmb2, E030044M24Rik, Myh9, Myhn 1, Myhn1, TU72.6

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Galerie der Validierungsdaten

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  WB analysis using 14844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14844-1-AP (Myh9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14844-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14844-1-AP (Myh9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14844-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14844-1-AP (Myh9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 14844-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 14844-1-AP (Myh9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

14844-1-AP bindet in WB, IHC, IF, ELISA Myh9 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy polypeptide 9, non-muscle
• Berechnetes Molekulargewicht: 226 kDa
• Beobachtetes Molekulargewicht: 224 kDa
• GenBank-Zugangsnummer: NM_022410
• Gene symbol: Myh9
• Gene ID (NCBI): 17886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH9, also named as NMMHC-A and NMMHC-IIA, is a cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. It plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction, during cell spreading.

Publikationen

Species	Application	Title
human, mouse	WB, IF	Nat Commun; The non-muscle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells.; Authors - Bing Zhao
mouse	IF, WB	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo ; KD Validated
human	WB	Cell Cycle; PMID: 23974104; Authors - Tyler J W Robinson
mouse	WB,IHC	Neuromolecular Med; Generation and characterization of mice with Myh9 deficiency.; Authors - Mhatre Anand N AN ; KO Validated
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
rat	WB	Biomed Pharmacother; Tandem mass tag (TMT) proteomic analysis of fetal lungs revealed differential expression of tight junction proteins in a rat model of congenital diaphragmatic hernia.; Authors - Xue Li