MYOF Rekombinanter Antikörper

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• KD/KO Validated

MYOF Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230213E10

Kat-Nr. : 82917-2-RR

Synonyme

Myoferlin, 230213E10, Fer 1 like family member 3, Fer 1 like protein 3, FER1L3

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  WB analysis using 82917-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82917-2-RR (Myoferlin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 82917-2-RR

  WB result of MYOF antibody (82917-2-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYOF transfected A549 cells.

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  IF Staining of HepG2 using 82917-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYOF antibody (82917-2-RR, Clone: 230213E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82917-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYOF Recombinant antibody (82917-2-RR, Clone:230213E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82917-2-RR bindet in WB, IF/ICC, FC (Intra), ELISA MYOF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myoferlin
• Berechnetes Molekulargewicht: 235 kDa
• Beobachtetes Molekulargewicht: 230-240 kDa
• GenBank-Zugangsnummer: NM_133337
• Gene symbol: MYOF
• Gene ID (NCBI): 26509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOF, also named FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.