MYH2-specific Monoklonaler Antikörper
MYH2-specific Monoklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1F1B6
Kat-Nr. : 66212-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | humanes Skelettmuskelgewebe |
Erfolgreiche Detektion in IHC | humanes Skelettmuskelgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:100000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 5 publications below |
IHC | See 1 publications below |
Produktinformation
66212-1-Ig bindet in WB, IHC, ELISA MYH2-specific und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | myosin, heavy chain 2, skeletal muscle, adult |
Berechnetes Molekulargewicht | 223 kDa |
Beobachtetes Molekulargewicht | 200 kDa |
GenBank-Zugangsnummer | NM_017534 |
Gene symbol | MYH2 |
Gene ID (NCBI) | 4620 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for MYH2-specific antibody 66212-1-Ig | Protokoll herunterladen |
IHC protocol for MYH2-specific antibody 66212-1-Ig | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Agric Food Chem Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice. | ||
Sci Rep Trimetazidine and exercise provide comparable improvements to high fat diet-induced muscle dysfunction through enhancement of mitochondrial quality control. | ||
Sci Rep Age-related thinning of orbicularis oculi muscle inside upper eyelid and its possible association with sunken upper eyelids | ||
J Biol Chem O-GlcNAcylation modification of MyoD regulates skeletal muscle fiber differentiation by antagonizing the UPF1 pathway | ||
J Muscle Res Cell Motil RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging | ||
Heliyon Light-phase time-restricted feeding disrupts the muscle clock and insulin sensitivity yet potentially induces muscle fiber remodeling in mice |