MYH2-specific Monoklonaler Antikörper

MYH2-specific Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1F1B6

Kat-Nr. : 66212-1-Ig

Synonyme

Myosin 2, MYH2, 1F1B6, MYHSA2, MyHC-IIa



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Skelettmuskelgewebe
Erfolgreiche Detektion in IHChumanes Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:100000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 5 publications below
IHCSee 1 publications below

Produktinformation

66212-1-Ig bindet in WB, IHC, ELISA MYH2-specific und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name myosin, heavy chain 2, skeletal muscle, adult
Berechnetes Molekulargewicht 223 kDa
Beobachtetes Molekulargewicht 200 kDa
GenBank-ZugangsnummerNM_017534
Gene symbol MYH2
Gene ID (NCBI) 4620
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MYH2-specific antibody 66212-1-IgProtokoll herunterladen
IHC protocol for MYH2-specific antibody 66212-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

J Agric Food Chem

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Authors - Luchu Zhang
WB

Sci Rep

Trimetazidine and exercise provide comparable improvements to high fat diet-induced muscle dysfunction through enhancement of mitochondrial quality control.

Authors - Wenliang Zhang
humanIHC

Sci Rep

Age-related thinning of orbicularis oculi muscle inside upper eyelid and its possible association with sunken upper eyelids

Authors - Takamasa Gomi
humanWB

J Biol Chem

O-GlcNAcylation modification of MyoD regulates skeletal muscle fiber differentiation by antagonizing the UPF1 pathway

Authors - Lele Kou
mouseWB

J Muscle Res Cell Motil

RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging

Authors - Runqing Shi
WB

Heliyon

Light-phase time-restricted feeding disrupts the muscle clock and insulin sensitivity yet potentially induces muscle fiber remodeling in mice

Authors - Zhou Ye