Myosin Light Chain 2/MLC-2V Monoklonaler Antikörper

Myosin Light Chain 2/MLC-2V Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5A1F1

Kat-Nr. : 60229-1-PBS

Synonyme

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

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  WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 60229-1-Ig (same clone as 60229-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 60229-1-Ig (same clone as 60229-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60229-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Myosin Light Chain 2/MLC-2V und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Myosin Light Chain 2/MLC-2V fusion protein Ag1356
• Vollständiger Name: myosin, light chain 2, regulatory, cardiac, slow
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC015821
• Gene symbol: Myosin Light Chain 2
• Gene ID (NCBI): 4633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.