N-cadherin Polyklonaler Antikörper

N-cadherin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(56)

Kat-Nr. : 13769-1-AP

Synonyme

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  WB analysis of HEK-293 using 13769-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13769-1-AP (N-cadherin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 13769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13769-1-AP (N-cadherin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13769-1-AP (N-cadherin Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 43 publications below
• IHC: See 9 publications below
• IF: See 6 publications below

Produktinformation

13769-1-AP bindet in WB, IHC, IF, ELISA N-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: N-cadherin fusion protein Ag4752
• Vollständiger Name: cadherin 2, type 1, N-cadherin (neuronal)
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 140 kDa
• GenBank-Zugangsnummer: BC036470
• Gene symbol: N-cadherin
• Gene ID (NCBI): 1000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

Publikationen

Species	Application	Title
		Biomaterials; Comparative effects of N-cadherin protein and peptide fragments on mesenchymal stem cell mechanotransduction and paracrine function.; Authors - Ellen C Qin
human	WB,IF	Theranostics; Gas6/Axl Axis Contributes to Chemoresistance and Metastasis in Breast Cancer through Akt/GSK-3β/β-catenin Signaling.; Authors - Cun Wang
mouse	WB	Int J Cancer; Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.; Authors - Chung-Chih Yang
mouse	WB	Oxid Med Cell Longev; Metformin Improves Fertility in Obese Males by Alleviating Oxidative Stress-Induced Blood-Testis Barrier Damage.; Authors - Jifeng Ye
human	WB,IHC	Oncotarget; microRNA-374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway.; Authors - Yiyu Chen
human	WB	Cancer Lett; Disrupting MALAT1/miR-200c sponge decreases invasion and migration in endometrioid endometrial carcinoma.; Authors - Qiulian Li