N-cadherin Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

N-cadherin Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(213)

CloneNo.

1D8B3

Rezensionen (3)

Kat-Nr. : 66219-1-Ig

Synonyme

1D8B3, Cadherin 2, Cadherin-2, CD325, CDH2

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  WB analysis of HEK-293 using 66219-1-Ig

  WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.

• 

  WB analysis using 66219-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66219-1-Ig

  Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of MKN-45 using 66219-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66219-1-Ig

  HEK-293 (N-cadherin+), PC-3 cells (N-cadherin+), C6 cells (N-cadherin+), NIH/3T3 cells (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66219-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66219-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66219-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 66219-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green.

• 

  IF Staining of mouse heart using 66219-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 66219-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 66219-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66219-1-PBS against Human N-cadherin were performed. The affinity constant is 12.9 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Kaninchenhirngewebe, C2C12-Zellen, C6-Zellen, HEK-293-Zellen, humanes Herzgewebe, MKN-45-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-3-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe, ROS1728-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes malignes Melanomgewebe, humanes Ovarialkarzinomgewebe, Maushirngewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:7500-1:30000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 179 publications below
• IHC: See 22 publications below
• IF: See 31 publications below

Produktinformation

66219-1-Ig bindet in WB, IHC, IF-P, ELISA N-cadherin und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: N-cadherin fusion protein Ag4996
• Vollständiger Name: cadherin 2, type 1, N-cadherin (neuronal)
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC036470
• Gene symbol: N-cadherin
• Gene ID (NCBI): 1000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

Publikationen

Species	Application	Title
monkey	IF	Cell Stem Cell; Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaques; Authors - Yongshun Lin
human	WB	Mol Cancer; YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.; Authors - Jiangfeng Li
human	WB	J Pineal Res; Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics; Authors - Ye Young Shin
human	WB,IHC	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
human	WB	Aging Cell; Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.; Authors - Liling Xiong
human	IF	Mater Today Bio; Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening.; Authors - Xinglong Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kiara (Verified Customer) (07-28-2025)

This primary antibody was not internally validated for IF, but there was a paper published that it worked on 4% PFA-fixed H9 ESCs, so I gave it a shot anyway. However, the staining did not show a nice membrane localization that our lab usually sees when using a different rabbit pan-cadherin primary antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: iPSC-derived cardiomyocytes

N-cadherin Antibody Immunofluorescence validation (1:400 dilution) in iPSC-derived cardiomyocytes (Cat no:66219-1-Ig)

FH

Davide (Verified Customer) (06-13-2023)

The antibody didn't produce any signal in 3d primary human cholangiocyte organoids. I would recommend either to try other antibody concentration or to try another Ab. It was tried on a cell condition which was recently reported to stimulate the CDH2 protein expression. However it should also be present in control condition at low levels.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cholangiocyte organoids

N-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:66219-1-Ig)

FH

Udesh (Verified Customer) (10-13-2022)

Used the Ab at 1:2000 dilution in 5% NFDM and observed a band at 140 kD. Blocked in 5% NFDM for 1H at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS Osteosarcoma cells

N-cadherin Antibody Western Blot validation (1:2000 dilution) in U2OS Osteosarcoma cells (Cat no:66219-1-Ig)