N-cadherin Monoklonaler Antikörper

N-cadherin Monoklonal Antikörper für WB, IF/ICC, FC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D9G1

Kat-Nr. : 68532-2-Ig

Synonyme

2D9G1, Cadherin 2, Cadherin-2, CD325, CDH2

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  WB analysis using 68532-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68532-2-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68532-2-Ig

  Immunofluorescent analysis of un-fixed HepG2 cells using N-cadherin antibody (68532-2-Ig, Clone: 2D9G1 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of HepG2 using 68532-2-Ig

  1x10^6 HepG2 cells were surface stained with 0.2 μg N-Cadherin Monoclonal Antibody (68532-2-Ig, Clone: 2D9G1, red) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue), and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Cells were not fixed.

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  FC experiment of SH-SY5Y using 68532-2-Ig

  1x10^6 MCF-7 (red, dashed) and SH-SY5Y (red, filled) were surface stained with 0.2 μg N-cadherin Monoclonal antibody (68532-2-Ig, Clone: 2D9G1) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were not fixed.

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  Affinity and Kinetic Characterization of 68532-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 68532-2-PBS against Human N-cadherin were performed. The affinity constant is 2.69 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Herzgewebe, Mausherzgewebe, Kaninchenherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68532-2-Ig bindet in WB, IF/ICC, FC, ELISA N-cadherin und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: N-cadherin fusion protein Eg0701
• Vollständiger Name: cadherin 2, type 1, N-cadherin (neuronal)
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_001792.4
• Gene symbol: N-cadherin
• Gene ID (NCBI): 1000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).