N-cadherin Rekombinanter Antikörper

N-cadherin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

230259D7

Kat-Nr. : 98006-1-RR

Synonyme

N-cadherin/CD325, 230259D7, Cadherin 2, Cadherin-2, CD325

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  FC experiment of HeLa using 98006-1-RR

  1x10^6 HeLa cells (left) or MCF-7 cells (right) were surface stained with 0.25 ug Anti-Human N-cadherin/CD325 Rabbit Recombinant Antibody (98006-1-RR, Clone: 230259D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98006-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98006-1-RR against Human N-cadherin were performed. The affinity constant is 2.73 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98006-1-RR bindet in FC N-cadherin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: N-cadherin fusion protein Eg0701
• Vollständiger Name: cadherin 2, type 1, N-cadherin (neuronal)
• Berechnetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_001792.4
• Gene symbol: N-cadherin
• Gene ID (NCBI): 1000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).