N4-acetylcytidine Monoklonaler Antikörper

N4-acetylcytidine Monoklonal Antikörper für Dot Blot, ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

chemical compound

Anwendung

Dot Blot, ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C2C2

Kat-Nr. : 68498-1-PBS

Synonyme

C11H15N3O6, Ac-rC, Acetylcytidine, ACETYL CYTOSINE, Ac4C

---

• 

  ELISA experiment of N4-acetylcytidine using 68498-1-Ig (same clone as 68498-1-PBS)

  Indirect ELISA and competitive ELISA results show that this antibody is specific to ac4C (N4-acetylcytidine). Indirect ELISA (blue curve, refer to top X-right Y axis) was performed by coating BSA conjugated ac4C at 50ng/well followed by blocking with 5% non fat milk. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Ac4C or its structure analogue compounds was mixed in 20ng/mL primary antibody (red curve, refer to bottom X-left Y axis). This data was developed using the same antibody clone with 68498-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of HeLa using 68498-1-Ig (same clone as 68498-1-PBS)

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 5% milk and blotted with N4-acetylcytidine antibody 68498-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG1 isotype control Monoclonal antibody 66360-1-Ig at the same dose. This data was developed using the same antibody clone with 68498-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68498-1-PBS bindet in Dot Blot, ELISA, Indirect ELISA N4-acetylcytidine und zeigt Reaktivität mit chemical compound

• Getestete Reaktivität: chemical compound
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Verbindung
• Vollständiger Name: N4-acetylcytidine
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N4-Acetylcytidine, CasNo. 3768-18-1, is a modified nucleoside and endogenous urinary nucleoside product of the degradation of tRNA, 18s rRNA and mRNA. N4-Acetylcytidine is a biological marker for various cancers with elevated concentrations present in urine. N4-Acetylcytidine is also a partially protected cytidine and therefore can be used as a synthetic building block to prepare further derivatized nucleosides such as 2',3'-dideoxycytidine. NAT10 catalyzes the formation of N4-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68498-1-IgDotBlot.pdf