N4BP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

N4BP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 16733-1-AP

Synonyme

KIAA0341, Nedd4 binding protein 3, NEDD4-binding protein 3

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  WB analysis using 16733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16733-1-AP (N4BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16733-1-AP

  IP result of anti-N4BP3 (IP:16733-1-AP, 4ug; Detection:16733-1-AP 1:2000) with mouse brain tissue lysate 1800 ug.

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  IHC staining of human kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 16733-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HEK-293 using 16733-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Zervixkarzinomgewebe, humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

16733-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA N4BP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: N4BP3 fusion protein Ag10194
• Vollständiger Name: Nedd4 binding protein 3
• Berechnetes Molekulargewicht: 544 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC053323
• Gene symbol: N4BP3
• Gene ID (NCBI): 23138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

Publikationen

Species	Application	Title
human	WB,IP	Front Microbiol; N4BP3 Regulates RIG-I-Like Receptor Antiviral Signaling Positively by Targeting Mitochondrial Antiviral Signaling Protein.; Authors - Chen Wang ; KD Validated
human	WB,IP,IHC	Cell Death Discov; N4BP3 facilitates NOD2-MAPK/NF-κB pathway in inflammatory bowel disease through mediating K63-linked RIPK2 ubiquitination; Authors - Wang Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (05-25-2023)

I used this antibody to assess protein levels in human T-ALL cells by western blot. I got 2 unspecific bands (40 and ~200 kDa). Signal very strong. Dilution: 1:1000 with 50 ug of lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell line