NACA Polyklonaler Antikörper

NACA Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32235-1-PBS

Synonyme

Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1

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  WB analysis of HeLa using 32235-1-AP (same clone as 32235-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 32235-1-AP (NACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

32235-1-PBS bindet in WB, IHC, Indirect ELISA NACA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NACA fusion protein Ag37704
• Vollständiger Name: nascent polypeptide-associated complex alpha subunit
• Berechnetes Molekulargewicht: 215 aa, 23 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC106041
• Gene symbol: NACA
• Gene ID (NCBI): 4666
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769).