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NADK Polyklonaler Antikörper

NADK Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29290-1-PBS

Synonyme

EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

Filter:
  • Western Blot (WB) analysis of various lysates using NADK Polyclonal antibody (29290-1-AP)

    WB analysis using 29290-1-AP (same clone as 29290-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NADK Polyclonal antibody (29290-1-AP)

    WB analysis of HEK-293 using 29290-1-AP (same clone as 29290-1-PBS)

    WB result of NADK antibody (29290-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-NADK transfected HEK-293 cells. This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NADK Polyclonal antibody (29290-1-AP)

    IHC staining of human stomach cancer using 29290-1-AP (same clone as 29290-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NADK Polyclonal antibody (29290-1-AP)

    IHC staining of human ovary cancer using 29290-1-AP (same clone as 29290-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29290-1-PBS bindet in WB, IHC, Indirect ELISA NADK und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NADK fusion protein Ag30982
Vollständiger Name NAD kinase
Berechnetes Molekulargewicht 49 kDa
Beobachtetes Molekulargewicht 49 kDa
GenBank-ZugangsnummerBC001709
Gene symbol NADK
Gene ID (NCBI) 65220
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).