NAGS Polyklonaler Antikörper

NAGS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 21566-1-AP

Synonyme

AGAS, Amino-acid acetyltransferase, ARGA, EC:2.3.1.1, N-acetylglutamate synthase conserved domain form

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  WB analysis using 21566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21566-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21566-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21566-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21566-1-AP (NAGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21566-1-AP (NAGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21566-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 21566-1-AP

  Immunofluorescent analysis of Hela cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

21566-1-AP bindet in WB, IHC, IF/ICC, ELISA NAGS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAGS fusion protein Ag16073
• Vollständiger Name: N-acetylglutamate synthase
• Berechnetes Molekulargewicht: 534 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58-66 kDa
• GenBank-Zugangsnummer: BC111713
• Gene symbol: NAGS
• Gene ID (NCBI): 162417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
mouse,human	WB,IHC	Heliyon; Machine learning deciphers the significance of mitochondrial regulators on the diagnosis and subtype classification in non-alcoholic fatty liver disease; Authors - Bingyu Wang
rat	WB	Drug Des Devel Ther; The Potential Mechanisms of Althaea rosea (Linn.) Cavan. Flower in Alleviating Tetrodotoxin Poisoning: An Integrated Metabolomics, Network Pharmacology and Experimental Validation; Authors - Renjin Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Heaji (Verified Customer) (09-30-2019)

As shown in the image the antibody recognizes a strong band of the wrong size in the NAGS knockout model -- I don't think this works as intended. Thank you.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver and intestine

NAGS Antibody Western Blot, validation (1:2000 dilution) in mouse liver and intestine (Cat no:21566-1-AP)