NAMPT/PBEF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NAMPT/PBEF Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Rezensionen (3)

Kat-Nr. : 11776-1-AP

Synonyme

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

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Galerie der Validierungsdaten

• 

  WB analysis using 11776-1-AP

  Rat liver lysates were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11776-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 11776-1-AP

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11776-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11776-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11776-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11776-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF(IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:4000) with mouse heart tissue lysate 1560 ug.

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11776-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of U-87 MG using 11776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, HL-60-Zellen, Mausherzgewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe, Ramos-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, HeLa-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 35 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Produktinformation

11776-1-AP bindet in WB, IP, IHC, IF, ELISA NAMPT/PBEF und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAMPT/PBEF fusion protein Ag2434
• Vollständiger Name: nicotinamide phosphoribosyltransferase
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC020691
• Gene symbol: NAMPT
• Gene ID (NCBI): 10135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala
human,mouse	IHC,WB	Cell Metab; NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.; Authors - Hongwei Lv ; KD Validated
mouse	WB,IHC	Cell Death Dis; Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy; Authors - Yang Deng
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
human,mouse	IF,WB	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li ; KD Validated
human	WB	Oncogenesis; Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.; Authors - Anna E Gibson ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

DAN (Verified Customer) (03-11-2022)

Very effective in getting targeted efficacy.

• Applications: Western Blot
• Cell Tissue Type: Human, Mouse, Rat

FH

kishu (Verified Customer) (06-10-2021)

this Ab works well for WB (1:100) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: mouse liver tissues and rat liver

FH

Iru (Verified Customer) (05-30-2019)

This antibody works really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: fallopian tube epithelial cells

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