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NAMPT/PBEF Monoklonaler Antikörper

NAMPT/PBEF Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

3D4D8

Kat-Nr. : 66385-1-Ig

Synonyme

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN



Geprüfte Anwendungen

Erfolgreiche Detektion in WBLNCaP-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, MOLT-4-Zellen, Maus-Skelettmuskelgewebe, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen, PC-3-Zellen, Rattenherzgewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66385-1-Ig bindet in WB, IHC, IF/ICC, ELISA NAMPT/PBEF und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen NAMPT/PBEF fusion protein Ag2434
Vollständiger Name nicotinamide phosphoribosyltransferase
Berechnetes Molekulargewicht 52 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC020691
Gene symbol NAMPT/PBEF
Gene ID (NCBI) 10135
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NAMPT/PBEF antibody 66385-1-IgProtokoll herunterladen
IHC protocol for NAMPT/PBEF antibody 66385-1-IgProtokoll herunterladenl
IF protocol for NAMPT/PBEF antibody 66385-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB,IF

Cell Metab

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer

Authors - Yu Jiang
human,mouseWB

Cell Chem Biol

NAMPT-targeting PROTAC and nicotinic acid co-administration elicit safe and robust anti-tumor efficacy in NAPRT-deficient pan-cancers

Authors - Xiaotong Zhu
mouseIF

Adv Biol (Weinh)

NAMPT/NAD+ /PARP1 Pathway Regulates CFA-Induced Inflammatory Pain via NF-κB Signaling in Rodents

Authors - Yi Dai
humanWB,IHC

J Cell Physiol

Nicotinamide phosphoribosyl transferase regulates cell growth via the Sirt1/P53 signaling pathway and is a prognosis marker in colorectal cancer.

Authors - Jing-Hua Pan
  • KD Validated
humanWB

Cell Rep

NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.

Authors - Yuetong Wang
humanWB

Cell Chem Biol

Addressing the Enzyme-independent tumor-promoting function of NAMPT via PROTAC-mediated degradation

Authors - Xiaotong Zhu