NAMPT/PBEF Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NAMPT/PBEF Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

3D4D8

Kat-Nr. : 66385-1-Ig

Synonyme

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

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Galerie der Validierungsdaten

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  WB analysis using 66385-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66385-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of human heart using 66385-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66385-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66385-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 66385-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66385-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 66385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 66385-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66385-1-Ig(NAMPT/PBEF antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, K-562-Zellen, Maus-Skelettmuskelgewebe, MCF-7-Zellen, MOLT-4-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen, PC-3-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

66385-1-Ig bindet in WB, IHC, IF, ELISA NAMPT/PBEF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NAMPT/PBEF fusion protein Ag2434
• Vollständiger Name: nicotinamide phosphoribosyltransferase
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC020691
• Gene symbol: NAMPT
• Gene ID (NCBI): 10135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Publikationen

Species	Application	Title
human	WB,IHC	J Cell Physiol; Nicotinamide phosphoribosyl transferase regulates cell growth via the Sirt1/P53 signaling pathway and is a prognosis marker in colorectal cancer.; Authors - Jing-Hua Pan ; KD Validated
human	WB	Cell Rep; NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.; Authors - Yuetong Wang
human	WB	Cell Chem Biol; Addressing the Enzyme-independent tumor-promoting function of NAMPT via PROTAC-mediated degradation; Authors - Xiaotong Zhu
mouse	WB	Int J Mol Sci; Regulation of NAD+/NADH Redox Involves the Protective Effects of Ginsenoside Rb1 against Oxygen-Glucose Deprivation/Reoxygenation-Induced Astrocyte Lesions; Authors - Ying Liu