NAMPT/PBEF Monoklonaler Antikörper

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• KD/KO Validated

NAMPT/PBEF Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D4D8

Kat-Nr. : 66385-1-PBS

Synonyme

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

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  WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66385-1-Ig (same clone as 66385-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66385-1-Ig (same clone as 66385-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66385-1-Ig (same clone as 66385-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 66385-1-Ig (same clone as 66385-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66385-1-Ig (same clone as 66385-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66385-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NAMPT/PBEF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NAMPT/PBEF fusion protein Ag2434
• Vollständiger Name: nicotinamide phosphoribosyltransferase
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC020691
• Gene symbol: NAMPT/PBEF
• Gene ID (NCBI): 10135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.