Nanog Polyklonaler Antikörper

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• KD/KO Validated

Nanog Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(283)

Rezensionen (4)

Kat-Nr. : 14295-1-AP

Synonyme

hNanog

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• 

  WB analysis using 14295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14295-1-AP (Nanog antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14295-1-AP (Nanog antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human embronic stem cells using 14295-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 14295-1-AP at dilution of 1:200. The PE shows staining with 14295-1-AP/PE. The DAPI shows nuclear staining by DAPI.

• 

  FC experiment of NICCT using 14295-1-AP

  1X10^6 NCCIT cells were intracellularly stained with 0.4 ug Anti-Human Nanog (14295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausembryogewebe, NCCIT-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: humane embryonale Stammzellen
• Erfolgreiche Detektion in FC (Intra): NCCIT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 191 publications below
• IHC: See 23 publications below
• IF: See 96 publications below

Produktinformation

14295-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Nanog und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nanog fusion protein Ag5645
• Vollständiger Name: Nanog homeobox
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC160187
• Gene symbol: Nanog
• Gene ID (NCBI): 79923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publikationen

Species	Application	Title
		Cell Stem Cell; A combined cell and gene therapy approach for homotopic reconstruction of midbrain dopamine pathways using human pluripotent stem cells.; Authors - Niamh Moriarty
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
human	IF	Cell Stem Cell; A cloaked human stem-cell-derived neural graft capable of functional integration and immune evasion in rodent models; Authors - Chiara Pavan
mouse	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
mouse		Mol Cell; RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.; Authors - Xianju Bi
mouse	IF	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chunxu (Verified Customer) (05-27-2025)

This antibody works very well in WB and IF to determine the stemness either cancer stem cells or mouse embryonic stem cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SKOV3, mESCs

FH

Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: mESCs

Nanog Antibody Western Blot validation (1-1000 dilution) in mESCs (Cat no:14295-1-AP)

FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

Nanog Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:14295-1-AP)

FH

Ying (Verified Customer) (08-19-2019)

very good antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse primary cardiomyocytes