Nanog Monoklonaler Antikörper

Nanog Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

CloneNo.

3A2E1

Kat-Nr. : 67255-1-Ig

Synonyme

hNanog, 3A2E1

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  WB analysis using 67255-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67255-1-Ig (Nanog antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HuH-7-Zellen, humanes Plazenta-Gewebe, JAR-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, NCCIT-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IF: See 1 publications below

Produktinformation

67255-1-Ig bindet in WB, IF, ELISA Nanog und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nanog fusion protein Ag21364
• Vollständiger Name: Nanog homeobox
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC160187
• Gene symbol: Nanog
• Gene ID (NCBI): 79923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma; Authors - Yongzhi Zhao
human	WB	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	WB,IF	Dent Mater; Microspheres of stem cells from human exfoliated deciduous teeth exhibit superior pulp regeneration capacity; Authors - Jianzhao Ding
human	WB	iScience; Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.; Authors - Xin Chen
human	WB	Biomed Pharmacother; Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.; Authors - Ling Gao
human	WB	Med Sci Monit; PMID: 31760402; Authors - Xueni Liu