Nanog Rekombinanter Antikörper

Nanog Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250067F10

Kat-Nr. : 80210-13-PBS

Synonyme

hNanog

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  WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02157-2

  Sandwich ELISA standard curve of MP02157-2, Human Nanog Recombinant Matched Antibody Pair - PBS only. 80210-13-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5645. 80210-12-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP02157-2

  The mean Nanog concentration was determined to be 9.25 ng/mL in NCCIT cell extract based on a 1.50 mg/mL extract load.

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  IHC staining of mouse brain using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  IF Staining of NCCIT using 80210-13-RR (same clone as 80210-13-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Nanog antibody (80210-13-RR, Clone: 250067F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02157-2

  Cytometric bead array standard curve of MP02157-2, Nanog Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80210-13-PBS. Detection antibody: 80210-12-PBS. Standard: Ag5645. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 80210-13-RR

  Biolayer interferometry (BLl) kinetic assays of 80210-13-RR against Human Nanog were performed. The affinity constant is 11.0 nM.

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Geprüfte Anwendungen

Produktinformation

80210-13-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Nanog und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nanog fusion protein Ag5645
• Vollständiger Name: Nanog homeobox
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC160187
• Gene symbol: Nanog
• Gene ID (NCBI): 79923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)