NANS Polyklonaler Antikörper

NANS Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 15163-1-AP

Synonyme

N-acetylneuraminic acid phosphate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminate synthase, N acetylneuraminate synthase, EC:2.5.1.57

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  WB analysis of HeLa using 15163-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15163-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15163-1-AP

  IP result of anti-NANS (IP:15163-1-AP, 4ug; Detection:15163-1-AP 1:500) with HepG2 cells lysate 3300 ug.

• 

  IHC staining of human stomach cancer using 15163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15163-1-AP (NANS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15163-1-AP (NANS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

15163-1-AP bindet in WB, IHC, IP, ELISA NANS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NANS fusion protein Ag7271
• Vollständiger Name: N-acetylneuraminic acid synthase
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC000008
• Gene symbol: NANS
• Gene ID (NCBI): 54187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The sialic acids are a family of 9-carbon 2-keto-3-deoxy sugars. They are frequently the terminal sugars on secreted and cell surface glycoproteins and glycolipids. Sialic acids participate in many important biologic recognition events. NANS can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Publikationen

Species	Application	Title
human,mouse	IHC	Cell Mol Gastroenterol Hepatol; The Role of the Hexosamine-Sialic Acid Metabolic Pathway Mediated by GFPT1/NANS in c-Myc-Driven Hepatocellular Carcinoma; Authors - Shiguan Wang
human	IHC	Exp Cell Res; Reanalysis of single-cell data reveals macrophage subsets associated with the immunotherapy response and prognosis of patients with endometrial cancer; Authors - Qianhua Wu
Human		Front Immunol; Identification and verification of the optimal feature genes of ferroptosis in thyroid-associated orbitopathy; Authors - Xuemei Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: LUNG Fibroblast

NANS Antibody Western Blot validation ( dilution) in LUNG Fibroblast (Cat no:15163-1-AP)