NAP1;NCKAP1 Rekombinanter Antikörper

NAP1;NCKAP1 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242784C5

Kat-Nr. : 85142-2-PBS

Synonyme

NCKAP1, 242784C5, HEM2, KIAA0587, Membrane-associated protein HEM-2

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  WB analysis using 85142-2-RR (same clone as 85142-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85142-2-RR (NAP1;NCKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85142-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01841-2

  Sandwich ELISA standard curve of MP01841-2, Humanc NCKAP1 Recombinant Matched Antibody Pair - PBS only. 85142-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2788. 85142-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

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  Sample test of MP01841-2

  The mean NCKAP1 concentration was determined to be 38.80 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 46.44 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP01841-2

  Cytometric bead array standard curve of MP01841-2, NCKAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85142-3-PBS. Detection antibody: 85142-2-PBS. Standard: Ag2788. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 85142-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85142-2-RR against Human NAP1;NCKAP1 were performed. The affinity constant is 0.116 nM.

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Geprüfte Anwendungen

Produktinformation

85142-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test NAP1;NCKAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NAP1;NCKAP1 fusion protein Ag2788
• Vollständiger Name: NCK-associated protein 1
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 115-125 kDa
• GenBank-Zugangsnummer: BC015025
• Gene symbol: NCKAP1
• Gene ID (NCBI): 10787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.