NAP1;NCKAP1 Rekombinanter Antikörper

NAP1;NCKAP1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242784C5

Kat-Nr. : 85142-2-RR

Synonyme

NCKAP1, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

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  WB analysis using 85142-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85142-2-RR (NAP1;NCKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85142-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85142-2-RR against Human NAP1;NCKAP1 were performed. The affinity constant is 0.116 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85142-2-RR bindet in WB, ELISA NAP1;NCKAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NAP1;NCKAP1 fusion protein Ag2788
• Vollständiger Name: NCK-associated protein 1
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 115-125 kDa
• GenBank-Zugangsnummer: BC015025
• Gene symbol: NCKAP1
• Gene ID (NCBI): 10787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.