NAPB Polyklonaler Antikörper

NAPB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14106-1-PBS

Synonyme

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  WB analysis using 14106-1-AP (same clone as 14106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  IP result of anti-NAPB (IP:14106-1-AP, 4ug; Detection:14106-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14106-1-AP (same clone as 14106-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using NAPB antibody 14106-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14106-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NAPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAPB fusion protein Ag5239
• Vollständiger Name: N-ethylmaleimide-sensitive factor attachment protein, beta
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC060840
• Gene symbol: NAPB
• Gene ID (NCBI): 63908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.