NAPRT1 Monoklonaler Antikörper

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NAPRT1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5D8H10

Kat-Nr. : 66159-1-PBS

Synonyme

NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21

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  WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 cells using 66159-1-Ig (same clone as 66159-1-PBS)

  WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66159-1-Ig (same clone as 66159-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66159-1-Ig (same clone as 66159-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66159-1-PBS bindet in WB, IHC, IF/ICC, ELISA NAPRT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NAPRT1 fusion protein Ag4265
• Vollständiger Name: nicotinate phosphoribosyltransferase domain containing 1
• Berechnetes Molekulargewicht: 514 aa, 55 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC032466
• Gene symbol: NAPRT1
• Gene ID (NCBI): 93100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).