NARG1 Polyklonaler Antikörper

NARG1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23845-1-PBS

Synonyme

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  WB analysis of HeLa using 23845-1-AP (same clone as 23845-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23845-1-AP (same clone as 23845-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23845-1-AP (same clone as 23845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 23845-1-AP (same clone as 23845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 23845-1-AP (same clone as 23845-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23845-1-AP (NARG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23845-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NARG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NARG1 fusion protein Ag20842
• Vollständiger Name: NMDA receptor regulated 1
• Berechnetes Molekulargewicht: 866 aa, 101 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC093928
• Gene symbol: NARG1
• Gene ID (NCBI): 80155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NAA15 (N-alpha-acetyltransferase 15) is a subunit of both the NatA and NatE complexes, and its role is to position the catalytic subunits in close vicinity to the nascent polypeptides (PMID: 33557580). Human NAA15 encodes an 866 amino acid (~105 kDa) protein, NAA15, containing tetratricopeptide repeat domains and a putative bipartite nuclear localization signal (PMID: 29656860). NAA15 was implicated in human disease and loss-of-function NAA15 variants in large cohorts of patients with autism spectrum disorder and/or ID (PMID: 33103328).