NARS Polyklonaler Antikörper

NARS Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14882-1-AP

Synonyme

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

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  WB analysis using 14882-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14882-1-AP (NARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14882-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14882-1-AP (NARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14882-1-AP

  IP result of anti-NARS (IP:14882-1-AP, 4ug; Detection:14882-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver cancer using 14882-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14882-1-AP (NARS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14882-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14882-1-AP (NARS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14882-1-AP bindet in WB, IP, IHC, ELISA NARS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NARS fusion protein Ag6656
• Vollständiger Name: asparaginyl-tRNA synthetase
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC001687
• Gene symbol: NARS
• Gene ID (NCBI): 4677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj