NARS Monoklonaler Antikörper

NARS Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G5G12

Kat-Nr. : 67711-1-PBS

Synonyme

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

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  WB analysis using 67711-1-Ig (same clone as 67711-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67711-1-Ig (NARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67711-1-Ig (same clone as 67711-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67711-1-Ig (NARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67711-1-Ig (same clone as 67711-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NARS antibody (67711-1-Ig, Clone: 4G5G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67711-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NARS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NARS fusion protein Ag6656
• Vollständiger Name: asparaginyl-tRNA synthetase
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC001687
• Gene symbol: NARS
• Gene ID (NCBI): 4677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).