NASP Polyklonaler Antikörper

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• KD/KO Validated

NASP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 11323-1-AP

Synonyme

PRO1999, FLB7527

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• 

  WB analysis of mouse testis using 11323-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11323-1-AP (NASP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11323-1-AP

  IP result of anti-NASP (IP:11323-1-AP, 5ug; Detection:11323-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human testis using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11323-1-AP (NASP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11323-1-AP (NASP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lymphoma using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11323-1-AP (NASP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11323-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NASP antibody 11323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11323-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA NASP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NASP fusion protein Ag1824
• Vollständiger Name: nuclear autoantigenic sperm protein (histone-binding)
• Berechnetes Molekulargewicht: 788 aa, 85 kDa
• Beobachtetes Molekulargewicht: 150 kDa, 70 kDa
• GenBank-Zugangsnummer: BC010105
• Gene symbol: NASP
• Gene ID (NCBI): 4678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.

Publikationen

Species	Application	Title
human,mouse	WB	Genes Dev; The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.; Authors - Drané Pascal P
human	WB	Nat Struct Mol Biol; The program for processing newly synthesized histones H3.1 and H4.; Authors - Campos Eric I EI ; KD Validated
human	WB	J Cell Sci; Role of Template Activating Factor-I as a chaperone in linker histone dynamics.; Authors - Kato Kohsuke K
human,mouse,rat	WB,IHC	J Biol Chem; MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP).; Authors - Ma Wubin W
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
human	WB	Int J Nanomedicine; Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity.; Authors - Qing Yuan