NAT10 Rekombinanter Antikörper

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NAT10 Rekombinant Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5B9

Kat-Nr. : 82585-1-PBS

Synonyme

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

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  WB analysis using 82585-1-RR (same clone as 82585-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  WB result of NAT10 antibody (82585-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 82585-1-RR (same clone as 82585-1-PBS)

  IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82585-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82585-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA NAT10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NAT10 fusion protein Ag4184
• Vollständiger Name: N-acetyltransferase 10 (GCN5-related)
• Berechnetes Molekulargewicht: 1025 aa, 116 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC035558
• Gene symbol: NAT10
• Gene ID (NCBI): 55226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).