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NAT12 Polyklonaler Antikörper

NAT12 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25149-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using NAT12 Polyclonal antibody (25149-1-AP)

    WB analysis using 25149-1-AP (same clone as 25149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25149-1-AP (NAT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25149-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NAT12 Polyclonal antibody (25149-1-AP)

    IF Staining of U2OS using 25149-1-AP (same clone as 25149-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NAT12 antibody (25149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25149-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25149-1-PBS bindet in WB, IF/ICC, Indirect ELISA NAT12 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NAT12 fusion protein Ag18435
Vollständiger Name N-acetyltransferase 12 (GCN5-related, putative)
Berechnetes Molekulargewicht 362 aa, 39 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC118589
Gene symbol NAT12
Gene ID (NCBI) 122830
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa.