NBCe2 Polyklonaler Antikörper

NBCe2 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26150-1-AP

Synonyme

NBC4, NBCe2, SLC4A5

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  IHC staining of human liver cancer using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26150-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NBCe2 antibody (26150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26150-1-AP bindet in WB, IHC, IF/ICC, ELISA NBCe2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NBCe2 fusion protein Ag23558
• Vollständiger Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
• Beobachtetes Molekulargewicht: 108-127 kDa
• GenBank-Zugangsnummer: BC109221
• Gene symbol: SLC4A5
• Gene ID (NCBI): 57835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NBCe2, also named as SLC4A5 and NBC4, belongs to the anion exchanger (TC 2.A.31) family. NBCe2 mediates electrogenic Na+:HCO3 − transport, and can operate in both a 1:2, and 1:3 manner, but the transport stoichiometry as well as directionality have not been defined for the renal NBCe2. The expression of NBCe2 has been reported in nearly all kidney nephron segments and CDs, indicating that some species-specificity might be present (PMID: 32547422). NBCe2 has 8 isoforms with the molecular mass of 108-127 kDa.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma; Authors - Marina Giannaki
mouse	WB	Neuron; The sodium-bicarbonate cotransporter Slc4a5 mediates feedback at the first synapse of vision; Authors - Rei Morikawa